tyrZ [2019-03-13 17:00:27]

alternative tyrosyl-tRNA synthetase, required to prevent misincorporation of D-Tyr into proteins

Locus: BSU38460

Isoelectric point: 6.66

Molecular weight: 46.94 kDa

Protein length: 413 aa

Gene length: 1242 bp

Function: translation

Product: tyrosyl-tRNA synthetase (minor)

Essential: no

E.C.: 6.1.1.1

Synonyms: ipa-9r,tyrS1,tyrT

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → Aminoacyl-tRNA synthetases
• Groups of genes → Phosphoproteins → Phosphorylation on a Thr residue

Gene

Coordinates: 3,947,158 3,948,399

Phenotypes of a mutant

• sensitive against D-Tyr PubMed
• a tyrS tyrZ double mutant is not viable PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr) (according to Swiss-Prot) ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr) (according to Swiss-Prot)
• TyrZ is highly specific for L-Tyr, prevents misincorporation of D-Tyr into proteins, less processive than TyrS PubMed

Protein family

• TyrS type 2 subfamily (according to Swiss-Prot), class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)

Paralogous protein(s)

• TyrS

Modification

• phosphorylated on Thr-311 PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: dtrR-tyrZ
  Description: PubMed

  Sigma factors

  • TyrZ: sigma factor, PubMed, in TyrZ regulon

  Regulatory mechanism

  • DtrR: repression, PubMed, in DtrR regulon
  • T-box: anti-termination, in T-box

  Regulation

  • induced by tyrosine limitation (T-box) PubMed

Biological materials

Mutant

• QB53532 (aphA3), available in Jörg Stülke's lab
• BKE38460 (tyrZ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTTCCTCCTTTTTTAC, downstream forward: _UP4_TAATCTGATATTCCGAAACG
• BKK38460 (tyrZ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTTCCTCCTTTTTTAC, downstream forward: _UP4_TAATCTGATATTCCGAAACG

References

Reviews

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Original publications

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